Choose agarose beads when you need maximum binding
capacity, large-scale prep, or lowest cost per milligram of protein.
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What are the differences between direct IP and indirect IP?
Criterion | Direct IP | Indirect IP |
Principle | The capture antibody is covalently cross-linked or directly conjugated to the beads before sample addition | The antibody is first incubated with the sample to form an antigen-antibody complex; then Protein A/G-conjugated beads are added to capture the complex |
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