U-Blot® Human Nitrated Alpha-synuclein (Tyr125/133) Quantitative ELISA Kit

Name: U-Blot® Human Nitrated Alpha-synuclein (Tyr125/133) Quantitative ELISA Kit
SKU: WT131E
Price: 9999 USD
Availability: InStock

$9,999.00

SKU: WT131E

Specification: 1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Stock: In stock

Product details, specifications, documents, and related products are available from the catalog record. Learn more

Product details Specifications Q & A Education

Product details, specifications, documents, and related products are available from the catalog record.

Product Overview

Catalog No.: WT131E
SKU: WT131E
Product Type: Other
Size: 1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Stock Status: In stock
Available Stock: 20
Minimum Order Quantity: 1

Storage & Compliance

Storage: -20°C
Research Use Only: Yes

Additional Specifications

Size: 1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Species: Human
Compatible Sample Types: Tissue Lysates, Cell Lysates
Accession Number: P37840
Gene Id: 6622
Gene Symbols: SNCA NACP PARK1
Protein Name / Synonyms: Alpha-synuclein (Non-A beta component of AD amyloid) (Non-A4 component of amyloid precursor) (NACP)
Solid Support: 96-well Microplate
Design Principle: Sandwich-based
Method Of Detection: Colorimetric
Research Area: Neuroscience, Post-Translational Modifications, Inflammation, Autophagy, Nitration, Parkinson's Disease, Aging
Modification: --
Shipping Type: 2‑8 ℃
Storage: -20°C
Kit Components: --
Additional Materials Required: Microplate reader capable of measuring absorbance at 450 nm;Protease and Phosphatase inhibitors;Precision pipettes to deliver 2 µL to 1 mL volumes;Adjustable 1-25 mL pipettes for reagent preparation;100 mL and 1 liter graduated cylinders;Log-log graph paper or computer and software for ELISA data analysis;Absorbent paper;Tubes to prepare positive control or sample dilutions;Distilled or deionized water
Assay Procedure Summary: Prepare all reagents, samples, and Positive Control as instructed. in the manual; Add 100 µL Standard to each well; Incubate 2.5 hours at room temperature or overnight at 4ºC with gentle shaking; Add 100 µL prepared detection antibody to each well; Incubate for 1 hour at room temperature with gentle shaking; Add 100 µL prepared HRP-conjugated anti-rabbit IgG concentrate solution; Incubate for 1 hour at room temperature with gentle shaking; Add 100 µL TMB One-Step Substrate Reagent to each well; Incubate 30 minutes at room temperature; Add 50 µL Stop Solution to each well; Read at 450 nm immediately.
Storage/Stability: The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.
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