U-Blot® Acetylated-Histone H2AX ELISA Kit

The Acetylated-Histone H2AX ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human, mouse and rat cell lysates. By determining Acetylated Histone H2AX protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human, mouse and rat Acetylated-Histone H2AX. An anti-pan Histone H2AX antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and Histone H2AX present in a sample is bound to the wells by the immobilized antibody. The wells are washed and rabbit anti- Acetyl Lysine antibody is used to detect Acetylated Histone H2AX. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Acetylated Histone H2AX bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.This assay platform allows rapid quantification of acetylated protein in various cell lysates and supports high-throughput screening of a large number of different cell lysate samples without the time-consuming and labor-intensive Western Blot analysis. It features minimal hands-on operation time, user-friendly workflow and a fully non-radioactive detection system, ensuring safe, efficient and convenient experimental operation.