- Target
- Atg9a
- Reactivity
- Human,Mouse,Rat
- Application
- WB,IHC,IF,IP,ELISA
- MW(Calculated)
- 94kD
- MW(Observed)
- 100-110kD
- Host Species
- Rabbit
- Isotype
- IgG, Kappa
- Conjugate/Modification
- Unmodified
- Modification site
- --
- Recommended Dilution Ratio
- IHC 1:200-1:1000;WB 1:10000-1:50000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200
- Form
- PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
- Source
- --
- Purification
- Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
- Purity
- --
- storageCondition
- -15°C to -25°C/1 year (Do not lower than -25°C)
- Concentration
- --
- Clonality
- Monoclonal
- Clone No.
- PT1883R
- Immunogen
- --
- Sequence
- --
- Specificity
- Endogenous
- Gene Name
- ATG9A APG9L1
- Protein Name
- Atg9a
- Other Name
- ATG9 autophagy related 9 homolog A;S. cerevisiae;
- Organism-1
- Human
- Gene ID-1
- 79065
- SwissProt-1
- Q7Z3C6
- Organism-2
- Mouse
- Gene ID-2
- --
- SwissProt-2
- Q68FE2
- Organism-3
- Rat
- Gene ID-3
- --
- SwissProt-3
- Q5FWU3
- Organism-4
- --
- gene ID-4
- --
- SwissProt-4
- --
- Cellular Localization
- Preautophagosomal structure membrane ; Multi-pass membrane protein . Cytoplasmic vesicle , autophagosome membrane ; Multi-pass membrane protein . Golgi apparatus , trans-Golgi network membrane ; Multi-pass membrane protein . Late endosome membrane ; Multi-pass membrane protein . Recycling endosome membrane ; Multi-pass membrane protein . Endoplasmic reticulum membrane ; Multi-pass membrane protein . Mitochondrion membrane ; Multi-pass membrane protein . Mainly localizes to the trans-Golgi network (TGN) and the endosomal system; cycles between them though vesicle trafficking (PubMed:27316455 , PubMed:27663665) . Export from the TGN to promote formation of autophagosomes is mediated by the AP-4 complex (PubMed:29180427 , PubMed:30262884) . Under amino acid starvation or rapamycin treatment , redistributes to preautophagosomal structure/phagophore assembly site (PAS) (PubMed:16940348) . The starvation-induced redistribution depends on ULK1 , ATG13 , as well as SH3GLB1 (PubMed:16940348) . Upon autophagy induction , a small portion transiently localizes to the autophagic membranes (PubMed:22456507) . Recruited to damaged mitochondria during mitophagy in a RIMOC1-dependent manner (PubMed:34432599) . .