- Target
- HSP27
- Reactivity
- Human,Mouse
- Application
- WB,IHC,IF,ELISA
- MW(Calculated)
- 23kD
- MW(Observed)
- 27kD
- Host Species
- Rabbit
- Isotype
- IgG,Kappa
- Conjugate/Modification
- Phospho
- Modification site
- --
- Recommended Dilution Ratio
- IHC 1:100-1:500;WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000;
- Form
- PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
- Source
- --
- Purification
- Protein A
- Purity
- --
- storageCondition
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Concentration
- --
- Clonality
- Monoclonal
- Clone No.
- PT1395R
- Immunogen
- --
- Sequence
- --
- Specificity
- HSP27 (Phospho Ser82) Antibody detects endogenous levels of HSP27 protein only when phosphorylated at S82.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):QLsSG
- Gene Name
- HSPB1 HSP27 HSP28
- Protein Name
- Heat shock protein beta-1
- Other Name
- HSPB1;HSP27;HSP28;Heat shock protein beta-1;HspB1;28 kDa heat shock protein;Estrogen-regulated 24 kDa protein;Heat shock 27 kDa protein;HSP 27;Stress-responsive protein 27;SRP27
- Organism-1
- Human
- Gene ID-1
- 3315
- SwissProt-1
- P04792
- Organism-2
- Mouse
- Gene ID-2
- 15507
- SwissProt-2
- P14602
- Organism-3
- Rat
- Gene ID-3
- 24471
- SwissProt-3
- P42930
- Organism-4
- --
- gene ID-4
- --
- SwissProt-4
- --
- Cellular Localization
- Cytoplasm . Nucleus . Cytoplasm, cytoskeleton, spindle . Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles. .