U-Blot® Topoisomerase I Rabbit mAb

U-Blot® Topoisomerase I Rabbit mAb
U-Blot® Topoisomerase I Rabbit mAb
$268.00
Size:50μL
SKU: WR4986-50

Catalog No.: WR4986

Specification: 50μL/100μL

Stock: In stock

U-Blot® Topoisomerase I Rabbit mAb Learn more

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Product information

Product detailsSpecificationsQ & AEducation

Background：This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22. [provided by RefSeq, Jul 2008],

Product Overview

Catalog No.
WR4986
SKU
WR4986-50
Category
Rabbit mAbs
Product Type
Other
Size
50μL/100μL
Stock Status
In stock
Available Stock
20
Minimum Order Quantity
1

Storage & Compliance

Storage
-15°C to -25°C/1 year(Do not lower than -25°C)
Research Use Only
Yes

Additional Specifications

Target
Topoisomerase I
Reactivity
Human,Mouse,Rat
Application
WB,IHC,IF,ELISA
MW(Calculated)
91kD
MW(Observed)
91kD
Host Species
Rabbit
Isotype
IgG,Kappa
Conjugate/Modification
Unmodified
Modification site
--
Recommended Dilution Ratio
IHC 1:200-1:1000;WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000;
Form
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Source
--
Purification
Protein A
Purity
--
storageCondition
-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
--
Clonality
Monoclonal
Clone No.
PT1218R
Immunogen
--
Sequence
--
Specificity
Endogenous
Gene Name
TOP1
Protein Name
DNA topoisomerase 1
Other Name
TOP1;DNA topoisomerase 1;DNA topoisomerase I
Organism-1
Human
Gene ID-1
7150
SwissProt-1
P11387
Organism-2
Mouse
Gene ID-2
21969
SwissProt-2
Q04750
Organism-3
Rat
Gene ID-3
64550
SwissProt-3
Q9WUL0
Organism-4
--
gene ID-4
--
SwissProt-4
--
Cellular Localization
Catalytic activity:ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.,Disease:A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with NUP98.,enzyme regulation:Specifically inhibited by camptothecin (CPT), a plant alkaloid with antitumor activity.,Function:The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another.,miscellaneous:Eukaryotic topoisomerase I and II can relax both negative and positive supercoils, whereas prokaryotic enzymes relax only negative supercoils.,miscellaneous:When a topoisomerase transiently breaks a DNA backbone bond, it simultaneously forms a protein-DNA link, in which a tyrosyl oxygen in the enzyme is joined to a DNA phosphorus at one end of the enzyme-severed DNA strand.,PTM:Sumoylated. Lys-117 is the main site of sumoylation. Sumoylation plays a role in partitioning TOP1 between nucleoli and nucleoplasm. Levels are dramatically increased on camptothecin (CPT) treatment.,similarity:Belongs to the eukaryotic type I topoisomerase family.,subcellular location:Diffuse nuclear localization with some enrichment in nucleoli. On CPT treatment, cleared from nucleoli into nucleoplasm. Sumolyated forms found in both nucleoplasm and nucleoli.,subunit:Monomer. Interacts with SV40 Large T antigen; this interactions allows viral DNA replication.,

Tech Support

ucallm

01

Documents

Datasheet, COA, SDS, and protocol files can be requested from technical support.

02

Storage & Handling

Storage
-15°C to -25°C/1 year(Do not lower than -25°C)
06

Contact Support

Emailinfo@mail.ucallm.com

Phone+(1)-866-986-9598

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Keywords:Rabbit mAbs