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In vivo transfection reagents are ready-to-use lipid nanoparticle kits that leverage the intrinsic physicochemical properties of the nanoparticles and the physiological features of the body to achieve non-specific organ enrichment without the need for targeting ligands. They deliver a broad range of nucleic acids—including ASO, siRNA, mRNA, and circRNA—with high efficiency in both animal models and cells.